What is ?
Anther culture is the process to obtain haploids plants (n chromosomal number) in invItro condition on a suitable medium. first anther culture technique was developed by Guha and Maheshwari in 1964. that way can be used is over 200 species of plant including barley, rice, tobacco, and more. first culture by this methods done in 1966 for Datura species of plant.
Requirement- For any anther culture in invtro condition require the nutritional medium, hormones, temperature, Suitable equipment’s, culture room with suitable conditions. etc.
Procedure- At the suitable stage of pollen development young flower bud uses for process. than surface sterilized this and rinsed with sterile water. than carefully transfer to anther on pertidish by used of dissection method for flower bud. than culture on them on agar medium or in liquid medium. after abut 24- 28 says anther burst open.than apply growth regulators like cytokinin, and auxin in appropriate ration and allow to culture after formation of plantlets in pots. applying them on open environment or field (soil).
Haploids produced in very large number with least of time. in some species that induces cell dissection in immature pollen. technique is very easy and frequently compare other methods.
Very low number of green plants obtain from this way. and some species showing high majority of non-haploid plants. many time anther got damage during its isolation. some times to achieved a individual response particular orientation is necessary in this process.
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