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                       BIOTECH TRANING, PROJECT, TOOLS & TECHNIQUES

 

 

 

 

 

                   



 

 

TRANING-

 

 

Why?

The aim of this  is to provide training to Biotech students for skill development and enhancing their job opportunities in biotech field. This type of traning  provides an opportunity to Biotech Industry for selecting suitable manpower.


Traning is most important requirement for all the students, researchers and personals interested in carrying out life science research. Biotechnology Training programs goal to train all the interested individuals to function as independent researchers in a multidisciplinary environment of Biotechnology,Forensic Science,and clinical Research etc. To achieve this aim instituets or companies have assembled a team of expert specializing in Bioinformatics/Biomedical,Molecular Biology,Biotechnology,Statistics and Forensic Science.  Forensic Scientists and Clinical Research professionals instead of merely a specialist in setting sample sizes and performing data analysis of simple experiments. Biotech training  is attending demand because of high promises in R&D ahead. Institutes or companies  providing biotechnology training and projects are back into focus creating awareness of practical implication of life sciences among students. these intitutes are of the leading institutes in biotechnology and bioinformatics training in world wide, lucknow. From various universities students seeking their final semester/final year Biotechnology project in there rely on for quality hands on training and project in Biotechnology, bioinformatics and related disciplines.


These follwing are some important instutes for biotechnology traning programme-



1.  Delhi

    National Research Centre on Plant Biotech | www.nrcpb.org

    Allele Life Sciences | www.allelelifesciences.com


    G Biosciences | www.gbiosciences.com

    IIT Delhi | www.iitd.ernet.in

    Imperial Life Sciences -  Gurgaon | http://imperialls.com



2. Chennai

    GeoMarine Biotechnologies | www.geomarinebiotech.com

    Jayagen | www.jayagen.com



3.Hyderabad

    Biomed Informatics | www.biomedlifesciences.com

    Ventura Institute of Biosciences | www.venturabio.com

    Nthrys | www.nthrys.com

    DRC | www.dnares.in

    Institute of Biotech | www.ushabiotech.com



4.Bangalore

    IBAB | www.ibab.ac.in/research_summer%20training.html

    Bhat Biotech | www.bhatbiotech.com

    Aristogene Biosciences | www.aristogene.com


5.Mumbai

    Rishi Biotech | www.rishibiotech.com/students/training.htm

 

 




                  
 

 

 

 

 

PROJECT-



A list of projects which have been published in several different national andInternational Reviewed Journals. list of the most successful projects  may be browsed in the publication/patent section. they (Companies And Istitutes) organize number of Biotechnology training programe, Bioinformaics workshops,  in various Universities and colleges in India. they has vast experience for organising different training programe in Biotechnology, Bioinformatics, Clinical Research, Forensic science, Microbiology, Immunology in many major cities. Selection of an Industrial and live project in biotechnology, clinical research, SAS, DNA Forensics and the related decipline is assistted by these laboratory experts so that a sudent may meet up the challenges of present Biotechnology and pharma industry.

 

 Explore the area of biotechnology with some of the following topics found in the science fair project ideas below:-



    1.Modern genetic engineering techniques (such as isolating and/or manipulating DNA)



    2.Chemical reactions and pathways that are important in living organisms



    3.Information about an organism based on DNA analysis.

 



The Imporatant Idea's Are-



1. Column Chromatography: Can you Separate the Dyes in Grape Soda Using Space Sand-


View

What the color is grape soda? If you pour it into a clear glass you can easily found  it is purple, but that is usually not its natural color. producers add red and blue dye in  to the soda. The dyes mix together and you find purple soda. What if you wanted to un-mix the dyes, could you? Yes! In a  lab, using a technique called column chromatography, you could isolate the two dyes again. But what about at home, can you use low-tech supplies to do the same thing? In this science project you will try to do just that using some interesting materials including a craft product called Space Sand.


Aim

Investigate whether a homemade column chromatography colum can be used to separate and isolate the different food colorings that are in grape soda.



2.. Forensic Science: Building Your Own Tool for Identifying DNA

View

When scientist want to isolate several different pieces of DNA, RNA, or proteins they use a technique known as gel electrophoresis. In this science project you'll  build a gel electrophoresis chamber and use it to found how many components are in different colors of food coloring dye.

Aim

In this  project you will build your own gel electrophoresis chamber and use it to compare molecules in different colors of food coloring dye.
 

 


3.Bioluminescence: Investigating Glow-in-the-Dark Dinoflagellates


View


Imagine waves glowing a beautiful blue color. The marine dinoflagellate Pyrocystis lunula is responsible for this magnificent phenomenon. Pyrocystis  lunula is a bioluminescent living organism —bioluminescence is the production of light by organisms. But does this organism always glow, no matter what the conditions, such as how much light there is? In this biotechnology science fair project, you will investigate how altering this dinoflagellate's exposure to light and dark affects its bioluminescence.

Aim

The aim of this biotechnology science fair project is to investigate how the bioluminescence of the marine dinoflagellate Pyrocystis lunula is affected by changes to its light-dark cycle.


4. Turn Plants into Biofuel with the Power of Enzymes

View

Are biofuels the souce of the energy in  the future? People says these plant-derived fuels as a way to someday cut down on our dependency on non-renewable carbon-based fuels, like gasoline. Ethanol (a type of alcohol) is a common biofuel used today. In the United States, ethanol is a common biofuel additive to normal gasoline. In fact, some states mandate that when you fill up your gas tank, 10 percent of the total fuel volume be made of ethanol. Brazil, the world's largest user of ethanol-based fuel, has been using ethanol biofuel to power cars since 1970.



5. Exploring DNA Damage: What Effect Do Ultraviolet Rays Have on Yeast Colony Growth?

View


The Sun provides heat and light, which are essential for life on Earth, ultraviolet (UV) rays in sunlight can create damage to DNA. In this science fair project, you will experiment with a strain of yeast that is super-sensitive to UV light. This project will demonstrate the lethal effects of UV light when DNA damage is not repaired.

 

Aim

Measure the lethal DNA effects of sunlight on the UV-sensitive yeast.

 

 

 



                  
 

 

 

 

 



TOOL AND TECHNIUES-



 

 

ENZYMES
 

 


 

RESTRICTION ENDONUCLEASE-

 

A restriction endonuclease is an enzyme that cuts DNA at on the specific recognition nucleotide sequences known as restriction sites. Restriction enzymes are commonly classified into three major  types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially.

 

 

                                                                                                                  

 

                                                                    

 

 

 

METHYLASE-



A  methylase is a type of transferase enzyme that transfers a methyl group from a donor to an acceptor. Methylation often occurs on nucleic bases in DNA or amino acids in protein structures. Methytransferases use a reactive methyl group bound to sulfur in S-adenosyl methionine as the methyl donor.

 


                                                                



DNA methylation is often used to silence and control genes without changing the original DNA sequence, an example of epigenetic modification. This methylation occurs on cytosine residues. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice, they caused a recessive lethal phenotype. Methylation may also be linked to cancer development, as methylation of tumor suppressor genes promotes tumorigenesis and metastasis.



LYGASE-


A DNA ligase is a specific type of enzyme, that facilitates the adding of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template with DNA ligase creating the final phosphodiester bond to fully repair the DNA.


DNA ligase has benifits in both DNA repair and DNA replication. In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments. Purified DNA ligase is used in gene cloning to join DNA  molecules together to form recombinant DNA.





 

VECTORS-



Definition:



When science use small pieces of DNA to clone a gene and create a genitically modified organisms  that DNA is called a vector. The vector serves as the carrier for the transfer or insertion of gene. Vectors are among the essential tools for gene cloning and are most useful if they also encode some kind of marker gene encoding abioindicator molecule that can be measured in a bioassay to ensure their insertion, and expression, in the host organism.In some cases, viruses are used to infect bacteria. These viruses are called bacteriophages, or phage, for short. Retroviruses are excellent vectors forintroducing genes into animal cells.

Plasmids, circular pieces of DNA, are generally used to introduce foreign DNA into bacterial cells. They often carry antibiotic resistance genes that can be used to test for expression of the plasmid DNA, on antibiotic petri plates. Gene transfer into plant cells is commonly performed using the soil bacterium Agrobacterium tumefaciens, which acts as a vector and inserts a large plasmid into the host cell.




Plasmids



In addition to the main chromosome, some bacteria contain small, accessory rings of DNA called plasmids.

Bacteria are capable of taking up plasmids from their environment. The genes on the plasmid are then expressed after it is taken up. This process is called transformation because the bacteria have new characteristics; they have been transformed. Foreign genes can be inserted into plasmids using genetic engineering technology. For example, the gene for human growth hormone has been put in plasmids and taken up by bacteria. The transformed bacteria secrete human insulin.

When the bacteria reproduce, the plasmids are also reproduced. The reproduction of genes that have been added to DNA is called cloning. The genes added to the plasmid have been cloned.

 

 

 

                                    

 

 

 

 



Viruses

Viruses are the vectors of choice for inserting genes into animal cells.

They can accept larger amounts of DNA than plasmids.When the virus reproduces within the animal cell, it also reproduces the foreign gene that it carries. The gene is therefore cloned.The cDNA of some retroviruses becomes integrated into the host chromosome.






GEL ETROPHTRESIS-



That is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.It is used in clinical chemistry to separate proteins by charge or size and  in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge. Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through an agarose matrix. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called  sieving. Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.

 

 

 

                                   

 

 

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